The invention discloses a 3D animation photo shooting device. An object end of the 3D animation photo shooting device comprises a support, a suspension bracket and a dial. The dial is horizontally fixed to the upper portion of the support. A shaft of the suspension bracket is movably connected to the upper portion of the support and penetrates through a center hole of the dial. A pointer is arranged on the portion, located above the dial, of the shaft in the radial direction. A suspension device for hanging a subject is arranged at the lower portion of the suspension bracket. A guide groove which can allow a camera to be arranged and allow the camera to slide is formed in the portion, at a shooting end of the 3D animation photo shooting device, of a machine frame. Positioning devices are arranged at the two ends of the guide groove. Sliding rails for limiting the sliding track of the camera are arranged on the two sides of the guide groove. By means of the 3D animation photo shooting device, a 3D animation photo can be accurately and truly shot, operation is easy, convenient and accurate, and the 3D animation photo shooting device is particularly suitable for shooting soft objects and objects with irregular shapes, for example, human tissue and organ specimens. The 3D animation photo shooting device can be used for manufacturing a short film, and therefore popularization of 3D animation or a 3D film is facilitated. The invention further provides application of the 3D animation photo shooting device for shooting the human tissue and organ specimens.