The invention provides an out-bottle cutting and rooting method for a sequoia sempervirens tissue culture seedling. The out-bottle cutting and rooting method comprises the following steps: taking and inserting a sterile sequoia sempervirens tissue culture multiplied seedling taken as a cutting slip into a base material composed of perlite, turf and red soil in a ratio of 1 to 1 to 2; putting the cutting slip into a greenhouse with the light shielding rate being 50-70%; keeping the air humidity to be more than 90% in one to two weeks after cottage is implemented at the temperature of 23+/-2 DEG C; keeping the humidity to be 60-80% within 30-40 days after two weeks to obtain a rooting seedling; transplanting the rooting seedling into a mixed base material of the perlite, humus and the red soil in a ratio of 1 to 1 to 2; putting the rooting seedling into the greenhouse with the light shielding rate being 50-70%; and growing until young leaves grow to obtain a sequoia sempervirens plant with a developed root system, a healthy and strong plant and the transplanting survival rate being more than 90%. The problems that the production period is long, the transplanting survival rate of the tissue culture seedling is low and the industrial production cannot be realized in the tissue culture process of sequoia sempervirens are solved.