The invention relates to a measuring method of a soluble saccharide. The method includes specific steps of: pretreating a sample, extracting the soluble saccharide with distilled water, developing and comparing color, making a standard curve, and calculating the content of the soluble saccharide. According to the method, different sample pretreating methods are selected according to different experiment conditions and measuring contents, so as to guarantee conformity of experiment conditions. Experiment steps are simplified, namely, the sampling weight is reduced and steps of centrifuging, collecting liquid supernatant, combining the liquid supernatant, achieving a constant volume, and the like are omitted. Exploration of extraction is performed in a scale test tube with a stopper, thus reducing the time and the cost. The method is particularly suitable for measurement of samples in large batches.