The invention discloses a rice high-temperature-resistant related gene OsZFP, a selection marker and a separating method of the related gene. The invention provides a separated rice endogenous high-temperature-resistant gene (OsZFP gene for short) and a coded polypeptide thereof, a preferred rice cell comprising the high-temperature-resistant gene or the coded polypeptide thereof and a preparation method of a plant cell. The invention further provides a novel method and a novel technology for breeding a high-temperature-resistant new crop variety, including a regulatory sequence relevant to high temperature resistance, a tightly-linked molecular marker for marking the high-temperature-resistant gene and a sequence of the molecular marker.