The invention relates to a method for removing fructus trichosanthis pulp by assist of a biological enzyme, belongs to the field of medicament producing and processing, and aims at solving the technical problem of providing a method for removing fructus trichosanthis pulp by assist of a biological enzyme, and use of amylase and cellulose for removing the fructus trichosanthis pulp. The method for removing the fructus trichosanthis pulp by assist of the biological enzyme comprises the following steps: putting fructus trichosanthis seeds with pulp into a biological enzyme solution; taking the fructus trichosanthis seeds to wash and dry after separating the fructus trichosanthis seeds from the pulp, so as to obtain the fructus trichosanthis seeds. Furthermore, the invention also discloses use of amylase and cellulose for removing pulp. The method for removing the fructus trichosanthis pulp by assist of the biological enzyme has the advantages of being simple in process, convenient to operate, low in cost, high in removal efficiency and the like.