The invention relates to a total-angle light emitting LED light source based on a light transmittance substrate and a packaging method of the total-angle light emitting LED light source. LED chips of the light source adhere to the light transmittance substrate through die attachment adhesive and connected with an electric conduction electrode through a wire. A primary packaging mixed powder colloid and a secondary packaging mixed powder colloid are located on the front face and the back face of the light transmittance substrate respectively, and the parts close to the edges of the two sides of the light transmittance substrate are in an arc shape. The packaging method of the light source includes the steps that the die attachment adhesive is used for fixing the LED chips on the light transmittance substrate to be baked; the wire is used for connecting the LED chips with the electric conduction electrode; the front face of the light transmittance substrate is coated with mixed powder adhesive points to be dried after self leveling, so that the primary packaging mixed powder colloid is obtained; after the light transmittance substrate is turned over, the back face of the light transmittance substrate is coated with mixed powder adhesive points to be dried after self-leveling, so that the secondary mixed powder colloid is obtained. The LED light source is good in color temperature evenness and controllability, and because the mixed powder colloids are in an arc shape on the edges of the light transmittance substrate, the phenomenon that dark spaces exist when the LED light source emits light is improved.