The invention discloses a high-voltage pulse power supply which comprises a low-voltage direct-current input power source, a pulse width modulation module, a boost transformer, a voltage-multiplying circuit module, a voltage and current feedback circuit module, a chopper circuit module, an isolation transformer, a system control module, a key and wireless remote control module and a nixie tube display module. The pulse width modulation module, the boost transformer, the voltage-multiplying circuit module and the voltage and current feedback circuit module form a switch power supply closed loop system. The high-voltage pulse power supply has the advantages that requirements of practical application can be met, power consumption in practical application is greatly reduced, manufacturing and use cost is low, breakdown voltage can be made into the form of a pulse, and voltage, current, frequency and duty radio of the pulse can be adjusted adaptively. The pulse power supply can serve as the high-voltage pulse power supply for application such as excitation, ignition and material ionization.