The invention provides a preparation process flow for health-care folium mori tea and relates to the technical field of health-care tea. According to the preparation process flow, Harpin protein and an exogenous amino acid are used for adjusting the folium mori growth process so as to increase the accumulation amount of secondary metabolism products of folium mori, improve the quality of folium mori fresh leaves and improve the processing performance; the folium mori is subjected to far-ultraviolet radiation pavement and microwave green removal; a substance change dynamic condition and the sensory quality of the folium mori in the pavement and green removal process are recorded; tests are carried out on obtained high-temperature oxidase according to different free states and immobilized states; a substance change dynamic condition and a quality forming mechanism in a folium mori tea processing method can be recorded. According to the preparation process flow, the content of special amino acids of the folium mori and the content of flavone of the folium mori are increased by adopting biological technological means; the bitter taste is reduced and the content of aqueous extracts of finished products is increased by exploring and using far ultraviolet and instantaneous high-pressure technologies and the like, and the resource utilization rate of tea leaves can be increased greatly.