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发明公开
CN103898146A 一种重组人血管内皮细胞生长因子A的制备方法 无效 - 撤回

一种重组人血管内皮细胞生长因子A的制备方法
摘要：
本发明涉及一种重组人血管内皮细胞生长因子A的制备方法，使用原核表达系统可溶性表达重组人血管内皮细胞生长因子A，其中，可溶性表达由重组人血管内皮细胞生长因子A与分子伴侣蛋白融合表达。本发明还涉及一种编码分子伴侣蛋白、重组人血管内皮细胞生长因子A的基因序列的重组质粒，将其转化至工程菌后可以得到可溶性表达的重组人血管内皮细胞生长因子A融合蛋白。本发明提供的重组人血管内皮细胞生长因子A的制备方法，在原核表达系统中可溶性大量表达出重组人血管内皮细胞生长因子，该方法产量高、蛋白收率高、工艺简便、便于放大、节约成本。
摘要（英）：

The invention relates to a method for preparing a recombined human vascular endothelial cell growth factor A. The recombined human vascular endothelial cell growth factor A is solubly expressed by virtue of a prokaryotic expression system, wherein soluble expression is fusion expression of the recombined human vascular endothelial cell growth factor A and a molecular chaperone protein. The invention also relates to a recombined plasmid of coded molecular chaperone protein and the gene sequence of the recombined human vascular endothelial cell growth factor A, wherein the recombined plasmid can be converted into engineering bacteria so as to obtain solubly expressed recombined human vascular endothelial cell growth factor A fusion protein. The method for preparing the recombined human vascular endothelial cell growth factor A can be used for solubly expressing a large quantity of recombined human vascular endothelial cell growth factors in the prokaryotic expression system, has high yield and protein yield, is simple and convenient, is convenient to amplify and can be used for saving the cost.

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