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发明公开
CN103898225A 一种鉴别烟粉虱隐种和白粉虱的引物及鉴别方法 失效 - 权利终止

一种鉴别烟粉虱隐种和白粉虱的引物及鉴别方法
摘要：
本发明涉及一种鉴别烟粉虱隐种和白粉虱的引物及鉴别方法。一种鉴别烟粉虱MEAM1隐种、烟粉虱MED隐种和白粉虱的引物，所述引物为一对，分别为SEQ？ID？NO.1和SEQ？ID？NO.2所示的核苷酸序列。本发明还涉及利用上述引物鉴别烟粉虱隐种和白粉虱的方法。本发明所述鉴别烟粉虱MEAM1隐种、烟粉虱MED隐种和白粉虱的引物能够扩增烟粉虱MEAM1隐种和烟粉虱MED隐种基因组DNA中的BtabCSP2基因，而对白粉虱基因组DNA没有扩增作用，为鉴定烟粉虱MEAM1隐种、烟粉虱MED隐种和白粉虱提供了简便稳定的分子标记，解决了现有烟粉虱MEAM1隐种、烟粉虱MED隐种和白粉虱鉴定较繁琐的问题。
摘要（英）：

The invention relates to a pair of primers and method of identifying a cryptic specie of bemisia tabaci and trialeurodes vaporariorum. The pair of primers of identifying the cryptic specie of bemisia tabaci MEAM1 (Middle East-Asia Minor one), the cryptic specie of bemisia tabaci MED (Mediterranean) and trialeurodes vaporariorum is respectively nucleotide sequences shown as SEQ ID NO. 1 and SEQ ID NO. 2. The invention further relates to a method of identifying the cryptic specie of bemisia tabaci and trialeurodes vaporariorum by the primers. The primers of identifying the cryptic specie of bemisia tabaci MEAM1, the cryptic specie of bemisia tabaci MED and trialeurodes vaporariorum can amplify BtabCSP2 genes in genomic DNAs of cryptic species of bemisia tabaci MEAM1 and MED but have no amplification effect on genomic DNA of trialeurodes vaporariorum. The primers provide simple, convenient and stable molecular markers for identifying the cryptic specie of bemisia tabaci MEAM1, the cryptic specie of bemisia tabaci MED and trialeurodes vaporariorum and solve the problem that an existing method of identifying the cryptic specie of bemisia tabaci MEAM1, the cryptic specie of bemisia tabaci MED and trialeurodes vaporariorum is tedious.

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