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发明公开
CN103901010A 一种含有水蛭或地龙成分的中药制剂的生物活性测定方法 无效 - 驳回 复审申请

一种含有水蛭或地龙成分的中药制剂的生物活性测定方法
摘要：
本发明公开了一种含有水蛭或地龙的中药制剂的抗凝生物活性测定方法，体外实验证明水蛭或地龙的提取液能够剂量相关的抑制凝血酶活性，因此本发明通过采用荧光共振能量转移(FRET)方法来测定凝血酶的抑制作用可反映含有水蛭或地龙药材成分的药物制剂的抗凝活性，方法简便快速，误差小，重现性高。
摘要（英）：

The invention discloses a biological activity testing method of a Chinese medicine preparation containing a component of leech or earthworm. In-vitro experiment shows that a leach or earthworm extract liquid can inhibit the thrombin activity in a dose-dependent manner, so that the inhibition function of thrombin can be tested by using a fluorescence resonance energy transfer (FRET) method, the anti-coagulation activity of a medicine preparation containing medicine components such as leach or earthworm can be reflected, and the method is simple, convenient, rapid, small in error and high in reproducibility.

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