The invention relates to a two-time cleaning processing method for tobacco stems. The method comprises the steps that the tobacco stems are cleaned for the first time, substances on the surfaces of the tobacco stems and substances influencing the physical quality and sensory quality are removed, and the moisture content of the tobacco stems is increased at the same time; the tobacco stems cleaned for the first time is postprocessed so as to meet the requirement of subsequent processing procedures; the tobacco stems which are cleaned for the first time and postprocessed are cleaned for the second time, and the substances on the surface of the tobacco stems and the substances influencing the physical quality and sensory quality are removed further, and the moisture content of the tobacco stems is further increased at the same time; the tobacco stems cleaned for the second time is postprocessed so as to meet the requirement of subsequent processing procedures. According to the two-time cleaning processing method for tobacco stems, due to the fact that the tobacco stems are cleaned twice, the tobacco stems are further humidified and expanded, moisture is sufficiently absorbed, harmful substances such as chloridion, phenolic substances and water soluble pectin on the surface of the tobacco stems and inside the tobacco stems are further removed, tobacco powder can be removed, and the physical quality and sensory quality of the stem silks are improved.