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发明公开
CN103923977A 温和气单胞菌的LAMP检测引物组及试剂盒 有权

温和气单胞菌的LAMP检测引物组及试剂盒
摘要：
本发明公开了一种温和气单胞菌LAMP检测引物组及检测试剂盒。包括一对外引物AS-F3和AS-B3，一对内引物AS-FIP和AS-BIP，以及一对环引物AS-LF和AS-LB，所述试剂盒包括检测引物组、BstDNA聚合酶、LAMP反应液、封闭液矿物油、阳性对照和阴性对照。还公开了利用上述温和气单胞菌的LAMP检测试剂盒检测温和气单胞菌的方法；本发明的试剂盒操作简便快速，适合现场检测；特异性强；检测灵敏度高，可达到320fg/mL；准确率高、可靠，具有广泛的应用前景。
摘要（英）：

The invention discloses an LAMP detection primer combination of aeromonas sobria and a kit. The LAMP detection primer combination of aeromonas sobria comprises a pair of outer primers AS-F3 and AS-B3, a pair of inner primers AS-FIP and AS-BIP and a pair of loop primers AS-LF and AS-LB. the kit comprises the detection primer combination, BstDNA polymerase, an LAMP reaction solution, confining liquid mineral oil, a positive control and a negative control. The invention also discloses a method for detecting aeromonas sobria by the utilization of the above LAMP detection kit of aeromonas sobria. The kit provided by the invention is simple and rapid to operate, is suitable for field test, has high specificity, high detection sensitivity and high and reliable accuracy, and has a wide application prospect. The detection sensitivity of the kit can reach 320fg/mL.

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