The invention provides a network performance measurement method, device and system. The method is applied to a measurement server in the network performance measurement system. The measurement system further comprises a network probe. The measurement method includes the steps that the measurement server responds to a network performance measurement request sent to a target server to send a data request to the network probe, and responds to a data packet with the designated length to send the network performance measurement request to the target server, wherein the data packet is returned by the network probe; the measurement server receives network performance measurement data returned by the target server; the measurement server measures the network performance of the target server according to the network performance measurement request and the network performance measurement data. The network performance measurement method, device and system solve the problems of client side calculation, storage, network bandwidth, power consumption and safety, and measurement of large-scale deployed networks is facilitated.