The invention discloses a feed regulating agent for producing low-cholesterol eggs and a feed containing the regulating agent. The feed regulating agent is prepared from a plurality of nutrients, a plurality of Chinese herbal medicine raw materials, metallothionein coarse powder and pine needle meal according to a certain ratio. The feed is prepared from the regulating agent and common feed raw materials according to a certain ratio. The feed regulating agent for producing the low-cholesterol eggs disclosed by the invention adopts a reasonable formula, has wide raw material source, and is low in cost, good in effect, simple and convenient to operate, and free of residue and pollution, and the method is easy to implement. No antibiotics and chemical medicine are added, the physiological function of a laying hen can be effectively improved, the immune function of the laying hen body can be enhanced, the egg yield and the egg quality of the laying hen are improved, especially the cholesterol content in the laying hen is obviously reduced, the culture benefits are improved, and the feed is especially applicable to large-scale production of the laying hen industry.