The invention discloses an anesthesia method for northern pike. The anesthesia method comprises the following steps: (1) adding a water resource with the same temperature as that of a parent fish pond in an anesthesia container, wherein the oxygen-dissolving value of the water source needed by anesthesia is more than or equal to 7mg/L; (2) adding an anesthetic into the water source obtained in the step (1), mixing uniformly, and then obtaining an anesthesia water source; and (3) placing the northern pike in the anesthesia water source and providing the application. The anesthesia method disclosed by the invention has the beneficial effects that by optimization of the relation between the concentration of 2-phenoxyethanol, the anesthesia time, the anesthesia water temperature and the anesthesia effect of the northern pike, the occurrence of the hyperanesthesia phenomenon can be effectively avoided, the time for egg collecting and semen collecting operation after the parent fish leaves from water in artificial propagation of the northern pike can be provided, and after enhanced breeding, the parent fish is repeatedly utilized next year, so that the collection scale of the fertilized egg and the utilization rate of the parent resource are increased, the dependence and the fishing intensity on natural resources are reduced and the purpose of sustainable development and utilization of the northern pike resource is achieved.