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发明公开
CN103952149A 一种二氯化-1,1′-二甲基-4,4′-联吡啶的荧光检测试剂及检测方法 失效 - 权利终止

一种二氯化-1,1′-二甲基-4,4′-联吡啶的荧光检测试剂及检测方法
摘要：
一种二氯化-1,1'-二甲基-4,4'-联吡啶的荧光检测试剂及检测方法，它涉及联吡啶除草剂的荧光检测试剂及方法。它是要解决现有的对百草枯的荧光检测方法中存在的检测物质难于获得、操作繁琐的技术问题。本发明的试剂是以荧光染料CXT为荧光分子探针。是由水、缓冲溶液和荧光染料CXT组成的溶液。定性检测是通过荧光检测试剂加入待测试样前后是否出现CXT的荧光猝灭来判断的；定量检测是通过标准曲线法来检测的。本发明荧光染料CXT作为荧光分子探针，可用于湖水及污水等实际环境水样品中的DBPD除草剂的检测。
摘要（英）：

The invention relates to a fluorescent detection reagent and a detection method for dichlorinated-1, 1'-dimethyl-4, 4'-dipyridyl, relating to a fluorescent detection reagent and method for a dipyridyl herbicide and aiming to solve the technical problems of difficulty in obtaining detection substances and complex operation in the traditional fluorescent detection method for paraquat. A fluorescent dye CXT is used as a fluorescent molecular probe in the reagent provided by the invention. The reagent is a liquid composed of water, a buffer liquid and the fluorescent dye CXT. Qualitative detection is realized through judging whether fluorescence quenching of CXT appears before and after the fluorescent detection reagent is added into a sample to be tested; and quantitative detection is realized by using a standard curve method. According to the invention, the fluorescent dye CXT is used as the fluorescent molecular probe, so that the fluorescent detection reagent can be used for detecting a DBPD herbicide in an actual environmental water sample such as lake water, sewage and the like.

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