The invention discloses a real-time full-high-definition2D-to-3D system line reading and writing time sequence design method. A line reading and writing time sequence is reasonably designed in the converting process, two pieces of Block RAM are added in an edge graph data-caching mechanism of a depth estimation system and a depth graph data-caching mechanism of the depth estimation system respectively, reading operation or writing operation can be distributed into different time areas, DDR2 is prevented from being read and written at the same time and can be normally read and written, performing of depth estimation and depth image drawing is guaranteed, and real-time full-high-definition 2D-to-3D normal running is guaranteed.