The invention belongs to the technical field of an aquatic feed additive, and particularly discloses a phagostimulant specially used for hybrid snakeheads and a preparation method of the phagostimulant. The phagostimulant comprises the following components by dry matter mass percent: 15-30% of compound Chinese herbal medicine extract, 34-51% of compound amino acid, 15-20% of a sulfur compound, 10-20% of alkaloid and 9-24% of nucleotide, wherein the compound Chinese herbal medicine extract is prepared from the following raw materials: radix astragali, cinnamon, peppermint, fructus forsythiae and sophora flavescens. A great number of creative tests prove that the phagostimulant has a synergetic effect by using the compound Chinese herbal medicine extract comprising the radix astragali, the cinnamon, the peppermint, the fructus forsythiae and the sophora flavescens together with the compound amino acid, the sulfur compound, the alkaloid and the nucleotide according to the specific formula, and is capable of remarkably improving the food calling effect of the hybrid snakeheads.