The invention relates to a precise two-dimensional motion occasion, can be used for precise displacement compensation of a general X-Y motion platform and particularly relates to a stiffness frequency adjustable two-dimensional micro-motion platform based on a stress stiffening principle. The stiffness frequency adjustable two-dimensional micro-motion platform based on the stress stiffening principle comprises X-direction and Y-direction motion sub-platforms, corresponding drivers and a micro-motion working platform, utilizes a film group for adjusting tensile force through screws as a flexible chain and achieves independent adjustment of vibrating frequency. By adopting the structure, the stiffness frequency adjustable two-dimensional micro-motion platform is based on prestress films, is adjustable in frequency and can adjust the inherent frequency of the micro-motion platform according to different working conditions and driving frequencies before working or in the working process, two feeding motion directions are orthogonal, and coupling of the micro-motion working table during two-dimensional motion is avoided.