A method for production of lactic acid bacteria by utilizing ethanol industrial yellow water includes collection and treatment of the ethanol industrial yellow water, cultivation of the lactic acid bacteria in the ethanol industrial yellow water, and receiving and preparation of the lactic acid bacteria. The method for production of the lactic acid bacteria by utilizing the ethanol industrial yellow water not only can make full use of the ethanol industrial yellow water, reduce the treatment cost of the yellow water, optimize comprehensive utilization of the yellow water and improve the ecological environment, but also can expand sources of a nutrition substrate in a conventional lactic acid bacteria production, lower the production costs and expand the application scope of lactic acid bacteria.