The invention discloses a sample carrier for ionization mass spectrometric analysis and an application thereof. The sample carrier is an egg shell which is treated to enable the inner surface to have a loose and porous structure. The treatment comprises the following steps: taking down the egg shell of a cooked poultry egg, firstly putting the egg shell into a 0.1-1mol/L hydrochloric acid aqueous solution, soaking at room temperature for 1-3 hours, then taking out the egg shell, cleaning hydrochloric acid on the egg shell by using water, and finally drying at the temperature of 30-40 DEG C for 3-6 hours. The sample carrier disclosed by the invention has the advantages that the egg shell is treated to enable the inner surface to have the loose and porous structure, so that the contact angle of the carrier with water and oil is enlarged; the sample carrier can be used as a sample carrier for MALDI-MS (Matrix-Assisted Laser Desorption Ionization Mass Spectrometry) and DESI-MS (Desorption Electrospray Ionisation-Mass Spectrometry) analysis, not only can the difficulty of spreading in sample application of the MALDI-MS and DESI-MS analysis be solved, but also the sensitivity of the analysis is improved, and the wasted egg shell can be turned into valuables, so that the sample carrier has practical value.