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发明公开
CN104012520A 一种抗氧化的牛冷冻精液稀释液及制作方法 无效 - 撤回

一种抗氧化的牛冷冻精液稀释液及制作方法
摘要：
本发明公开了一种抗氧化的牛冷冻精液稀释液，包括下列质量份的原料组成：三羟甲基氨基甲烷20~26，柠檬酸三钠10~15，果糖7~15，蒸馏水700~800，新鲜卵黄230~260，亚硒酸钠0.125~0.375，维生素E2~32，甘油90-95，注射用青霉素钾（规格为160万单位/g）1~2、注射用硫酸链霉素（规格为100万单位/g）1~2。制备方法包括制备基础缓冲液、制备基础稀释液、制备精液稀释液，然后将制备好的精液稀释液移至4℃条件下平衡5-20min，制得抗氧化的牛冷冻精液稀释液。本发明较现有技术的牛冷冻精液稀释液的精子活率提高了24.56个百分点，4h精子活率提高了12.39个百分点，精子顶体完整率提高了38.72个百分点，GSH－PX的活性提高了260%，精子畸形率则下降了5.49个百分点。
摘要（英）：

The invention discloses anti-oxidative cattle frozen semen diluent. The anti-oxidative cattle frozen semen diluent is composed of the following raw materials by mass: 20-26 parts of trihydroxy methyl-aminomethane, 10-15 parts of trisodium citrate, 7-15 parts of fructose, 700-800 parts of distilled water, 230-260 parts of fresh yolk, 0.125-0.375 part of sodium selenite, 2-32 parts of Vitamin E, 90-95 parts of glycerol, 1-2 parts of penicillin potassium for injection (specification of 1.6 million units/g) and 1-2 parts of streptomycin sulfate for injection (specification of 1.0 million units/g). The preparation method of the anti-oxidative cattle frozen semen diluent comprises the steps of preparing a basic buffer solution, a basic diluent and a semen diluent; then transferring the prepared semen diluent to a condition of 4 DEG C, and balancing for 5-20 min to obtain the anti-oxidative cattle frozen semen diluent. Compared with a conventional anti-oxidative cattle frozen semen diluent, the anti-oxidative cattle frozen semen diluent provided by the invention increases semen motility rate by 24.56%, increases 4 h semen motility rate by 12.39%, increases semen perforatorium integrity rate by 38.72%, increases GSH-PX activity by 260%, and reduces semen aberration rate by 5.49%.

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