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发明授权
CN104017863B 一种太子参药材或原植物的快速分子鉴别方法 有权

一种太子参药材或原植物的快速分子鉴别方法
摘要：
本发明公开了一种太子参药材或原植物的快速分子鉴别方法，包括以下步骤：利用由序列表中序列1所示序列设计合成的序列如序列表中序列2和序列表中序列3所示的引物对鉴别样品进行PCR反应；PCR产物的检测：PCR反应产物中加入SYBR Green I核酸荧光染料，混匀后在紫外光下观察，PCR反应产物产生绿色荧光的样品即为太子参药材或原植物，PCR反应产物未产生绿色荧光的样品即为其它植物材料。本发明可根据太子参药材或原植物的特异性DNA片段(其序列如序列表中序列1所示)设计得到的一组引物(其序列如序列表中序列2和序列表中序列3所示)快速、准确的鉴别出太子参药材或原植物，该方法重现性好，效果明显。
摘要（英）：

The invention discloses a rapid molecular identification method of a Radix Pseudostellariae medicinal material or an original plant. The method comprises the steps of carrying out PCR reaction on the to-be-identified sample by a set of primers which are designed and synthesized by the sequences shown in the SEQ ID No. 1 in a sequence table and have the sequences shown in the SEQ ID No. 2 and SEQ ID No. 3 in the sequence table; detecting PCR products: adding SYBR Green I nucleic acid fluorescent dye in the PCR reaction product, mixing and observing under ultraviolet light, the sample from which green fluorescence is generated is Radix Pseudostellariae medicinal material or an original plant and the sample from which no green fluorescence is generated is the other plant material. According to the invention, the Radix Pseudostellariae medicinal material or original plant can be quickly and accurately identified by designing a set of primers (of which the sequences are shown in the SEQ ID No. 2 and SEQ ID No. 3 in the sequence table) according to the specific DNA fragment (of which the sequences are shown in the SEQ ID No. 1 in the sequence table) of the Radix Pseudostellariae medicinal material or original plant, and the method has the advantages of good reproducibility and obvious effects.

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