The invention discloses a fine fertilizer application method for flue-cured tobaccos K326. Fine fertilizer application for the flue-cured tobaccos K326 is realized by measuring the alkali-hydrolyzale nitrogen content, the effective phosphorous content and the effective potassium content, calculating the using amount of nitrogen, and then calculating the using amount of phosphorous and the using amount of potassium according to a fertilizer application ratio to finally obtain the amount of nitrogen, phosphorous and potassium fertilizers by a base fertilizer and topdressing combination mode, so that a certain yield of high-quality tobaccos can be obtained. According to the fine fertilizer application method, the most economic fertilizer application amount can be calculated, so that loss of redundant nutrients can be reduced, and the cost can be lowered to a certain extent; on the premise that the yield and the quality of the flue-cured tobaccos K326 are not affected, a requirement on the growth of the flue-cured tobaccos K326 is met under the least using amount of nitrogen, phosphorous and potassium. The tobaccos obtained by the method provided by the invention are high in quality; chemical components in the tobaccos are coordinated; the flue-cured tobaccos are fresh in color, mesophyll tissues are dense, leaves are high in toughness, the oil content is large, and requirements of a cigarette enterprise can be met.