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发明公开
CN104046691A 一种花生根结线虫检测试剂盒 无效 - 撤回

一种花生根结线虫检测试剂盒
摘要：
本发明公开了一种花生根结线虫检测试剂盒，属于生物技术检测领域。本发明的技术方案是：一种花生根结线虫检测试剂盒，主要包括无Mg2+的10×PCR缓冲液，浓度为25mM的Mgcl2溶液，浓度为0.1mM的dNTP溶液，浓度5U/μl的taq酶溶液，浓度都为10μM的上游引物溶液和下游引物溶液，所述的上游引物溶液和下游引物溶液包含一堆特异性引物，所述的一对特异性义务的核苷酸序列分别为：上游引物5，-GTGAGGATTCAGCTCCCCAG-3，,下游引物5，-CAAGACCCAATGGCCATAGG-3，.解决现有技术花生根结线虫检测存在的灵敏度低、稳定性差、操作烦琐及污染环境等技术问题。
摘要（英）：

The invention discloses a kit for detecting Meloidogyne arenria and belongs to the field of biotechnical detection. According to the technical scheme, the kit for detecting Meloidogyne arenria comprises a Mg<2+>-free 10*PCR buffer solution, a MgCl2 solution of which the concentration is 25mM, a dNTP solution of which the concentration is 0.1mM and a taq enzyme solution of which the concentration is 5U/mu l as well as an upstream primer solution and a downstream primer solution of which the concentrations are both 10mu M, wherein the upstream primer solution and downstream primer solution comprise a pair of specific primers, the nucleotide sequences of the pair of specific primers respectively are as follows: the upstream primer 5, -GTGAGGATTCAGCTCCCCAG-3, and the downstream primer 5, -CAAGACCCAATGGCCATAGG-3. According to the invention, the technical problems of low sensitivity, poor stability, cumbersome operation and environmental pollution in the existing Meloidogyne arenria detection technology are solved.

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