The invention discloses a tissue culture and rapid propagation method of solanaceae lycium brevipes, and aims to solve the problem of propagation of introduced lycium barbarum. The method comprises the following steps: explant treatment, primary culture, subculture, rooting culture, hardening seedling and transplanting, the advantages of the method are as follows: A, only 6-benzyl amino adenine and indole-3-acetic acid are used for mixing during subculture of the solanaceae lycium brevipes, the effect of hormone accumulation on multi times of subculture can be greatly reduced, the cost is also reduced; B, peat and vermiculite in the ratio of 1:1 are used as a hardening seedling and transplanting matrix, the matrix is simple in ratio, light in mass, good in performance in transplanting test, and higher in transplanting survival rate; and C, the tissue culture and rapid propagation method plays a great role on late improvement and application of local Chinese wolfberry.