利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN104099265A 一种蒙氏假单胞菌SCUEC2菌株及其筛选方法和用途 无效 - 驳回

一种蒙氏假单胞菌SCUEC2菌株及其筛选方法和用途
摘要：
本发明提供了一种蒙氏假单胞菌(Pseudomonas monteilii)SCUEC2菌株，该菌株的保藏编号为：CCTCC NO：M2014134，该菌株的核苷酸序列如SEQ ID NO.1所示。该菌株的不仅能够运用于烟碱降解中，而且还能够加快烟叶成熟。本发明同时还提供了一种筛选分离上述菌株的方法，包括以下步骤：首先富集培养，然后分离纯化最后将分离纯化后的菌株按单菌落接种于烟碱培养基中，在室温下摇床培养1-3天后，测定烟碱培养基的发酵液中烟碱含量，根据烟碱降解率的大小来复筛菌株，从而得到蒙氏假单胞菌(Pseudomonas monteilii)SCUEC2菌株。
摘要（英）：

The invention provides a Pseudomonas monteilii SCUEC2 strain, which has a preservation number of CCTCC NO:M2014134 and a nucleotide sequence shown as SEQ ID NO.1. The strain not only can be applied to nicotine degradation, but also can accelerate tobacco leaf ripening. The invention also provides a method for screening and separation of the strain. The method comprises the steps of: firstly conducting enrichment culture, then carrying out separation and purification, and finally inoculating the separated and purified strain into a nicotine medium by single colony, performing shake cultivation at room temperature for 1-3d, then measuring the content of nicotine in the fermentation broth of the nicotine medium, and re-screening the strain according to the nicotine degradation rate, thus obtaining the Pseudomonas monteilii SCUEC2 strain.

0/0