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发明公开
CN104111331A 应用于检测菊科植物病毒病的抗体芯片和试剂盒及其方法 有权

应用于检测菊科植物病毒病的抗体芯片和试剂盒及其方法
摘要：
本发明涉及一种应用于菊科植物病毒病的筛查和诊断的抗体芯片、试剂盒及其方法。一种应用于检测菊科植物病毒病的抗体芯片，其主要特点在于，包括有基膜和在基膜上分别点制的危害菊科植物的7种病毒的特异性捕获抗体(一抗)，所述的7种病毒的捕获抗体(一抗)：抗-菜豆黄花叶病毒、抗-南芥菜花叶病毒、抗-菜豆萎蔫病毒、抗-黄瓜花叶病毒、抗-烟草脆裂病毒、抗-烟草环斑病毒、抗-番茄斑萎病毒；在基膜上还封装有3个阳性质控点P，点制为碱性磷酸酶标记的IgG和封装有3个阴性质控点N，点制为pH 9.2-9.8的碳酸盐缓冲液。本发明的优点是成功构建了检测菊科植物多种病毒的抗体芯片，可以高通量地对多个病原进行综合性检测。
摘要（英）：

The invention relates to an antibody chip for screening and diagnosing compositae plant virus diseases, and a kit and a method of the antibody chip. The antibody chip for screening and diagnosing compositae plant virus diseases is mainly characterized by comprising a base membrane and specific capture antibodies (primary antibodies) which are respectively pointed on the base membrane and used for capturing seven types of viruses with harm to the compositae plant, wherein the antibodies (primary antibodies) for capturing seven types of viruses have resistance to bean yellow mosaic virus, resistance to arabis mosaic virus, resistance to curtobacterium flaccumfaciens virus, resistance to cucumber mosaic virus, resistance to tobacco rattle virus, resistance to tobacco ringspot virus and resistance to tomato spotted wilt virus; three positive quality control points P, IgG pointed to be alkaline phosphatase mark, three negative quality control points N and a carbonate buffer solution pointed to reach a pH being 9.2-9.8 are also encapsulated on the base membrane. The antibody chip for screening and diagnosing compositae plant virus diseases has the advantages that the antibody chip for detecting a plurality of viruses of the compositae plant is successfully built, so that comprehensive detection is carried out on a plurality of pathogens at a high flux.

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