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发明公开
CN104136620A 用于氧化还原辅因子的酶再生的方法 无效 - 驳回

用于氧化还原辅因子的酶再生的方法
摘要：
公开了一种用于一锅法反应中的氧化还原辅因子NAD+/NADH和NADP+/NADPH的酶再生的方法。在该方法中，由于相同反应批次中进行的至少两种另外的酶催化氧化还原反应(产物形成反应)，两种氧化还原辅因子中的一种以其还原型获得，且另一种以其氧化型获得。所公开的方法的特征在于：a)在将被还原的辅因子再转化为其初始氧化型的再生反应中，氧气或通式R1C(O)COOH的化合物被还原，且b)在将被氧化的辅因子再转化为其初始还原型的再生反应中，通式为R2CH(OH)R3的化合物被氧化，化合物中的R1、R2和R3具有不同含义。
摘要（英）：

Disclosed is a method for enzymatically regenerating the redox confactors NAD+/NADH and NADP+/NADPH in a one-pot reaction. In said method, one of the two redox cofactors is obtained in the reduced form thereof while the other redox cofactor is obtained in the oxidized form thereof as a result of at least two additional enzymatically catalyzed redox reactions (product forming reactions) taking place in the same reaction batch. The disclosed method is characterized in that a) oxygen or a compound of general formula R1C(O)COOH is reduced during the regeneration reaction converting the reduced cofactor back into the original oxidized form thereof, and b) a compound of general formula R2CH(OH)R3 is oxidized during the regeneration reaction converting the oxidized cofactor back into the original reduced form thereof, R1, R2, and R3 in the compounds having different meanings.

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