The invention relates to an additive of meat food in the field of food processing, in particular to a meat tendering agent and application thereof. The tendering agent is prepared from the following materials in parts by weight: 8-12 parts of pawpaw pulp, 3-5 parts of pineapple pulp, 2-4 parts of glucose powder and 2-4 parts of sodium bicarbonate powder. In the tendering agent, the pawpaw pulp is chaenomeles speciosa pulp or papaya pulp. Meat products processed by using the tendering agent provided by the invention are fresh, tender and juicy, are easily digested and absorbed, and have multiple health-care efficacies. In addition, the materials are easily obtained, and the preparation method is simple, and is suitable for industrial and standard production.