The invention provides an electric bus charging pile safety protection system and a use method in the working process thereof, and belongs to the field of electric bus charging pile safety protection devices. The electric bus charging pile safety protection system comprises an electric bus charging bin opening charging safety protection device. The electric bus charging bin opening charging safety protection device is provided with a warning board and a storage battery. A warning symbol composed of LED lamps is arranged on the front surface of the warning board. The storage battery is connected to a charging power source. The warning board is of an arc shape, a warning symbol B, a warning symbol C and a piezoelectric ultrasonic generator are further arranged on the front surface of the warning board, a warning circuit is arranged on the rear surface of the warning board, and the warning symbol, the warning symbol B and the warning symbol C are connected to the positive electrode and the negative electrode of the storage battery through the warning circuit. Car paint can not be scratched; any objects which may have the electric shock hazard can be prevented from getting close to the electric bus charging pile safety protection system, and the electric bus charging pile safety protection system is reliable; the service life of the LED lamps is long; after charging is completed, automatic safety prompting can be provided.