The invention relates to polyester conductive fibers and provides graphene conductive polyester fibers having good conductivity and lasting static resistance and a preparation method thereof. Through surface sulfonic acid group functionalization of graphene, graphene particles have sizes less than 1 micrometer and have good dispersibility, graphene, carbon nanotubes and a surfactant are stirred and mixed according to a mass ratio of 1-5: 1-5: 1-5: 1 and then are dispersed to form conducting particles, the conducting particles are added into polyester powder, the mixture is processed to form graphene superconducting masterbatches by a twin-screw granulation technology, and the graphene conductive polyester fibers are prepared by a spinning technology adopting a double-component composite spinning method or a single-component blending spinning method. The graphene conductive polyester fibers have the characteristics of simple production processes, low energy consumption, low labor and mass production feasibility.