The invention discloses a preparation method of Coprinus comatus chewable tablets. The preparation method is characterized in that: Coprinus comatus is used as the main raw material and subjected to steps including pretreatment, enzyme treatment, homogenizing, spraying and drying, mixing, softening and granulating, tableting, drying, shaping and drying. The Coprinus comatus chewable tablets produced by the preparation method are convenient to eat and are lustrous. The chewable tablets can be directly eaten or eaten after the tablets are dissolved in drinking water, and can be conveniently taken and eaten. Moreover, the chewable tablets can not be deteriorated after long-term preservation, and simultaneously have the efficacies of Coprinus comatus such as reinforcement of human immunity, increasing of appetite, and promoting of digestion.