The invention discloses a squalene oil and coneflower soft capsule and a preparation method of the squalene oil and coneflower soft capsule. The squalene oil and coneflower soft capsule is composed of the following components in parts by weight: 50-80 parts of squalene oil, 10-40 parts of a coneflower extract, 2-6 parts of vitamin E, 1-4 parts of bee wax and 1-4 parts of soyabean lecithin. Auxiliary materials are uniformly mixed by a colloid mill to prepare the soft capsule. The health food disclosed by the invention is scientific and reasonable in formula, simple in preparation process, and safe, stable and effective; and the squalene oil and coneflower soft capsule has the function of enhancing the immunity and is suitable for people with low immunity to take.