Disclosed is a calibration method of a thermal resistance type hot-fluid sensor. The method comprises the following steps: a), arranging a heat conduction flat plate; b), respectively symmetrically installing a plurality of hot-fluid sensors and temperature sensors at the two sides of the heat conduction flat plate; c), each hot-fluid sensor respectively sending an acquired hot-fluid value q1, a hot surface temperature t0 and a hot surface temperature t1 to a hot-fluid-temperature inspection tour instrument; d), the hot-fluid-temperature inspection tour instrument calculating a standard hot-fluid value q0 according to a formula shown in the descriptions; e), according to a formula C=q0/q1, calculating a hot-fluid value correction coefficient C; and f), when an error between the hot-fluid value correction coefficients C for N continuous times does not exceed 1%, calculating an average value C<->of N correction coefficients C, and taking the average value as a final hot-fluid value correction coefficient for writing into the configuration file of the hot-fluid-temperature inspection tour instrument. According to the invention, a calibration result is accurate, the precision is high, the convenience and feasibility are high, the problem of incapability of accurately calibrating a hot-fluid instrument in building thermal operation detection is effectively solved, and the experiment efficiency and the reliability are improved.