The invention relates to a natural biological non-poisonous air-dispersing type mosquito-repellent incense and a manufacturing method thereof. The natural biological non-poisonous air-dispersing type mosquito-repellent incense comprises the following components in percentage by weight: 76-87% of rice husk ash, 1-2.5% of herba artemisiae annuae essential oil, 1-2.5% of folium eucalypti essential oil, 3-5% of natural essence, 2-4% of methylcellulose, 1-2% of folium artemisiae argyi powder, 3-5% of vegetable starch, and 2-3 parts of bay leaf essential oil. The natural biological non-poisonous air-dispersing type mosquito-repellent incense has the advantages that mosquito-repellent active ingredients and flavored substances contained in the natural biological non-poisonous air-dispersing type mosquito-repellent incense all come from natural plants, so that the natural biological non-poisonous air-dispersing type mosquito-repellent incense has no toxic or side effect on a human body, the problem that the rice husk ash pollutes the environment can be solved, the cost of the product can be greatly lowered, thus achieving the effects of excellent quality and reasonable price. Proved by repeated tests, the natural biological non-poisonous air-dispersing type mosquito-repellent incense is remarkable in mosquito-repellent effect and has light fresh scent, and the acceptability of people can achieve 90% through the sensory evaluation of people.