The invention discloses a negative pressure setting packer, and relates to a packer used in oil exploitation engineer operation, in particular to setting of an underground simulation oil extraction device. The negative pressure setting packer comprises a piston shaft, a flow limiting body and a cylinder barrel, one end of the piston shaft is integrally provided with a piston, the piston shaft is hollow, the flow limiting body is in threaded connection to one end of an opening of the piston shaft, the flow limiting body and the piston shaft are communicated, the piston shaft is sleeved with the cylinder barrel, a sealing ring is arranged between the flow limiting body and the cylinder barrel, the piston shaft is sleeved with the sealing ring, the two ends of the sealing ring are respectively and tightly in press fit with the piston shaft through the flow limiting body and the cylinder barrel, the sealing ring is an elastic pipe body, and pressure guide holes are formed in the positions, corresponding to the sealing ring, of the piston shaft. Steps are formed between the piston shaft and the piston, the free end of the cylinder barrel is limited through the steps, and pressure guide holes are formed in the positions, on the inner side of the cylinder barrel, of the piston shaft. Radial water outlet holes are formed in the lateral wall of the flow limiting body. The negative pressure setting packer is safe, reliable, simple and reasonable in structure and convenient to manufacture and install.