The invention discloses an agilawood identifying thin-layer chromatography. The agilawood identifying thin-layer chromatography comprises the following steps: A, preparing a test solution : taking 0.5g of agilawood test sample powder, adding 10-20mL of ethanol, performing ultrasonic treatment for 20-30 minutes, naturally cooling, filtering, and taking filtrate as the test solution; B, preparing a control solution : taking 0.5g of agilawood control sample, adding 10-20mL of ethanol, performing ultrasonic treatment for 20-30 minutes, naturally cooling, filtering, and taking filtrate as the control solution; C, performing thin-layer chromatography: sucking 10 microliters of the solutions prepared in the steps A and B respectively, dotting the solutions on the same silica gel G thin plate, developing with ethyl acetate-acetone as a developing agent, with the developing distance of 12cm, taking out, drying, and viewing in ultraviolet lamp, wherein fluorescent spots with the same color are developed at corresponding positions of a chromatogram of the control sample and a chromatogram of the test sample. The agilawood identifying thin-layer chromatography is stronger in operability and better in reproducibility; the obtained thin-layer chromatogram information is more comprehensive.