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发明公开
CN104212817A 玉米锌铁调控转运体ZmZIP5基因及其应用 审中-实审

玉米锌铁调控转运体ZmZIP5基因及其应用
摘要：
本发明公开了玉米锌铁调控转运体ZmZIP5基因及其应用。本发明从玉米中分离得到锌铁调控转运体ZmZIP5基因，其cDNA序列为SEQ ID No.1所示。亚细胞定位表明，该锌铁调控转运体定位在细胞质膜及内质网上，在植物细胞中起到吸收、转运和储存锌铁以及参与锌铁离子的解毒等作用。定时定量RT-PCR表达分析发现，ZmZIP5对于胚和胚乳的发育起到重要的调控作用。酵母互补实验表明，ZmZIP5具有转运锌铁的功能。本发明所分离的ZmZIP5基因对于调控植物吸收、转运和储存锌铁的能力，调控胚和胚乳的发育以及增加粮食作物籽粒中锌铁含量等方面具有重要的应用潜力。
摘要（英）：

The invention discloses a Zea mays zinc iron-regulated transporter ZmZIP5 gene and its applications. The Zea mays zinc iron-regulated transporter ZmZIP5 gene is separated from Zea mays, and the cDNA sequence of the gene is represented by SEQ ID No.1. Subcellular localization shows that the zinc iron-regulated transporter is localized in the plasma membrane and endoplasmic reticulum of a cell, has zinc and iron absorption, transportation and storage effects in plant cells, and particles in detoxification of zinc and iron ions. Timing quantitative RT-PCR expression analysis shows that the ZmZIP5 has an important regulation effect on the embryo and endosperm growth. Yeast complementation experiments show that each of the ZmZIP5 has a zinc and iron transportation function. The separated ZmZIP5 gene has important application prospects in the regulation of zinc and iron absorption, transportation and storage of plants, promotion of the embryo growth and increase of the content of zinc and iron in grains of food crops.

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