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发明公开
CN104212835A 一种乙型肝炎病毒感染的小鼠模型的构建方法 失效 - 权利终止 复审申请

一种乙型肝炎病毒感染的小鼠模型的构建方法
摘要：
本发明涉及生物技术领域，特别涉及一种乙型肝炎病毒感染的小鼠模型的构建方法及其用途。本发明提供一种高ALT乙型肝炎病毒感染的小鼠模型的构建方法，包括如下步骤：向Tlr2基因缺陷小鼠给药HBV基因组DNA质粒，从而构建HBV乙型肝炎病毒感染的小鼠模型。本发明所提供的小鼠模型构建过程相对简单，技术手段相对容易，构建成本低。此外，本发明所采用的HBV质粒载体本身不具有毒性，不会诱导小鼠的免疫应答，还能够避免了嵌合体小鼠模型中存在的重症免疫缺陷，且所得HBV感染模型具有更高的血清ALT水平和明显的肝脏炎症表现。
摘要（英）：

The invention relates to the technical field of biology and in particular relates to a method for constructing a hepatitis B virus (HBV) infected mouse model and application of the HBV infected mouse model. The invention provides a method for constructing a high-ALT (alanine transaminase) HBV infected mouse model. The method for constructing the HBV infected mouse model comprises the step of medicating HBV genome DNA plasmid to a Tlr2 gene-deficient mouse, so that the HBV infected mouse model is constructed. The mouse model provided by the invention is relatively simple to construct, relatively easy in technological means and low in construction cost; besides, the adopted HBV plasmid vector is non-toxic, can not induce immune response of the mouse and also can avoid serious immunodeficiency in a chimera mouse model, and the obtained HBV infected model has higher blood serum ALT level and obvious liver inflammation expression.

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