The invention relates to a preparation method of 3-amido-2-isopropyl thiophenecarboxylate. The preparation method is characterized in that the 3-amido-2-thiophenic acid isopropyl ester is obtained through hydrolysis reaction, acylating chlorination reaction and esterification reaction by taking 3-amido-2-thiophenecarboxylate as a raw material. The preparation method disclosed by the invention solves the problem that a method for effectively preparing 3-amido-2-isopropyl thiophenecarboxylate is lack at present and has the characteristics of easily available raw material, low cost, high product yield and is easy and convenient to react and easy to amplify, and industrialized production is realized.