The invention discloses application of jatrorrhizine hydrochloride in preparation of a drug for preventing and treating influenza, and belongs to the field of traditional Chinese medicine application. The application is implemented by the following technical scheme: firstly incubating influenza virus neuraminidase liquid from different subtypes and jatrorrhizine hydrochloride in an incubator at 37 DEG C for 30 minutes, adding a fluorogenic substrate buffer solution to the incubator to be detected, finding out the situation that jatrorrhizine hydrochloride can play an effective role on H1N1, H3N2 and influenza virus B, detecting anti-influenza action of jatrorrhizine hydrochloride on a cellular level, finding out the situation that jatrorrhizine hydrochloride can treat the cells infected H1N1 and H3N2, and at last detecting the influence of jatrorrhizine hydrochloride on a mouse influenza model. The study shows that when jatrorrhizine hydrochloride is added, the mouse weight reducing degree of the influenza infected mice can be obviously inhibited; jatrorrhizine hydrochloride can enable the mice to gradually recover to the health level and obviously lower the lung index of the influenza infected mice.