利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN104263688A 一种节杆菌SCUEC3菌株及其筛选方法和用途 无效 - 驳回

一种节杆菌SCUEC3菌株及其筛选方法和用途
摘要：
本发明提供了一种节杆菌SCUEC3(Arthrobacter sp SCUEC3)菌株，该菌株的保藏编号为：CCTCC NO：M2014402，该菌株的核苷酸序列如SEQ ID NO.1所示。该菌株的不仅能够运用于烟碱降解中，而且还能够加快烟叶成熟。本发明同时还提供了一种筛选分离上述菌株的方法，包括以下步骤：首先富集培养，然后分离纯化最后将分离纯化后的菌株按单菌落接种于烟碱培养基中，在室温下摇床培养1-3天后，测定烟碱培养基的发酵液中烟碱含量，根据烟碱降解率的大小来复筛菌株，从而得到节杆菌SCUEC3(Arthrobacter sp SCUEC3)菌株。
摘要（英）：

The invention provides an arthrobacter sp SCUEC3 strain. The preservation number of the strain is CCTCC NO: M2014402. A nucleotide sequence of the strain is shown as SEQ ID NO.1. The strain can be applied to nicotine degradation and can be used for accelerating maturing of tobacco leaves. Meanwhile, the invention also provides a method for screening and separating the strain. The method comprises the following steps: firstly, performing enrichment culture; secondly, performing separation and purification; finally, inoculating the separated and purified strain in a nicotine culture medium according to a single colony, performing shake cultivation at room temperature for 1-3 days, determining the content of nicotine in the fermentation liquor of the nicotine culture medium, and re-screening the strain according to the size of the nicotine degradation rate to obtain the arthrobacter sp SCUEC3 strain.

0/0