The invention relates to a bactericidal preparation, namely vagifresh lotion with a trade name of Yuanhe vagifresh lotion. The vagifresh lotion is prepared from the following crude drugs by weight: 5kg of Chinese violets, 5kg of rhizoma smilacis glabrae, 5kg of golden cypress, 5kg of cortex dictamni, 5kg of radix stemonae, 5kg of fructus kochiae, 5kg of radix sophorae flavescentis, 5kg of fructus cnidii, 0.2kg of menthol, 0.25kg of chlorhexidine acetate and 1kg of ethanol. The vagifresh lotion adopts water extraction or ultrasonic extraction. According to the detection of Jilin Sanitary Monitoring and Inspection Center on inhibiting and killing bacteria by the vagifresh lotion, the antibacterial effects are remarkable. The vagifresh lotion has an average antibacterial rate of 100.00% on staphylococcus aureus, escherichia coli, candida albicans, and the like, is free of irritation on skin. The vagifresh lotion is free of irritation on skin and vagina mucosa.