The invention discloses sinorhizobium SCAUs65 and application thereof. The strain is separated and purified from a fresh soybean nodulation; the strain is collected in the China center for type culture collection on March 14, 2014, the collection number of the strain is CCTCC NO: M2014084, and the strain is classified and named as sinorhizobium fredii. The strain is applied to the production of szechuan soybeans. The sinorhizobium SCAUs65 disclosed by the invention is an excellent broad-spectrum soybean rhizobium strain which has the advantages of high symbiotic nitrogen-fixing capacity, wide applicable range on szechuan soybean variety, inorganic phosphorus dissolving capacity, organic phosphorus dissolving capacity and potassium dissolving capacity and high stress resistance and has good matching affinity with szechuan main planted soybean variety. A soybean is increased in yield more than 38.9% by being inoculated with the sinorhizobium SCAUs65 without applying a nitrogen fertilizer in different ecological regions and achieves the difference to a significance level compared with a soybean which is not inoculated with the sinorhizobium SCAUs65.