The invention relates to a pipeline flow tracer test comprehensive device of a pressure bearing karst aquifer system. The pipeline flow tracer test comprehensive device is composed of an overflow trough for controlling the water level of the whole aquifer system, a loose sand trough containing even media, three seepage pipelines different in length in a trough body, observation holes and water outlet sampling holes, wherein the seepage pipelines are provided with micropores and maintain hydraulic connection with the loose sand layer. A tracer agent injection pipe hole and a control valve are arranged at the most front water inlet end of each pipeline. The water level of the water inlet end and the water outlet end of the overflow trough is used for controlling the changes of the underground flow field of the aquifer system of a simulation trough, and the dual-medium environment with even hole seepage and pipeline flow is formed. Water is supplied through a submersible pump in the test, an automatic collecting system is used for recording the water level of an aquifer, samples are regularly collected from the sampling holes, the peak value features of the changes of the concentration of the tracer agent in the karst pipeline flow in the submersible water (confined groundwater) karst aquifer system are obtained through tracer agent concentration curve features, and scientific basis is provided for finding the development law of karst channels in the underwater system in field carbonate areas.