The invention relates to the technical field of animal species screening, and concretely relates to a formula material for trapping wild lucilia sericata into ovipositing and a screening method for nicotine-resistant lucilia sericata specie. The formula material is composed of the following compositions in percent by weight: 70-90% of bran, 10-30% of imported fish meal, and a nicotine aqueous solution which possesses the mass concentration of 0.1%-0.2% and is used to adjust the humidity of the above formula material to be 60%. By directly employing a nicotine aqueous solution with proper concentration for compatibility with bran and imported fish meal, the obtained formula material successively masks the unpleasant smell and bitter taste of nicotine, and helps to effectively trap wild lucilia sericata into ovipositing, also because of the proper and definite content of nicotine, the lucilia sericata larva nicotine-resistant capability obtained by employing different batches of formula materials with same weight composition in a same trapping environment are guaranteed to be basically same, and stable screening effect is obtained.