The invention discloses a printing spectrum color-separating method facing to metamerism anti-fake. The method comprises the following steps: realizing the reasonable selection of metamerism anti-fake detection light source according to Pearson's correlation coefficient computation; modeling in high precision in combination with the color generation characteristic of the printing device through the color scientific correlation computation formula so as to realize the accurate preparation of a metamerism sample; obviously improving the efficiency and accuracy of the metamerism sample preparation by using a multi-level screening method. In the metamerism sample preparation aspect facing to the printing technology, the method disclosed by the invention is more accurate in comparison with the existing method, and the implementation is convenient. After determining the preparation flow the metamerism sample, the design and the production of the metamerism anti-fake product in the printing and the package field can be realized through the printing mode in combination with various pattern design.