The invention discloses a feed capable of improving the growth performance of broiler chicken as well as a preparation method and an application of the feed. The feed comprises the following components in percentage by weight: 0.05-0.15% of N-carbamylglutamic acid, micronutrients and an energy feed. The preparation method of the feed comprises the following steps: (1) removing impurities of the micronutrients and the energy feed in percentage by weight, airing and then crushing the micronutrients and the energy feed into 40-100 meshes; and (2) mixing and stirring the micronutrients and the energy feed crushed by the step (1) with N-carbamylglutamic acid in percentage by weight, and sieving to obtain a feed finished product. By virtue of the N-carbamylglutamic acid-containing feed produced by the method is capable of facilitating to endogenous angiogenesis of broiler chicken and synthesis of protein, improving the daily gain of the broiler chicken, reducing the ratio of feed to weight and further improving the growth performance of the broiler chicken.